Vol 7 Issue 1 January 2019-March 2019

Abstract: Wolbachia surface protein (WSP) is an Outer membrane protein of gram negative bacteria Wolbachia. WSP has shown its functional role in immune response, cell proliferation, pathogenicity and controlled cell death program. The WSP gene has previously been cloned, but the expression of the protein is low and the purification is time consuming. Therefore the E. coli WSP gene was cloned into the pET19b vector to improve the protein expression and simplify the purification. The WSP gene sequence was utilized to design forward and reverse oligonucleotide primers that were used to PCR the gene from Wolbachia genomic DNA. The primers were designed with NdeI or Xho 1 restriction sites on the 5’and 3’ terminus respectively. The PCR product was sequenced to confirm the identity of WSP. The gene was cloned into the expression vector pET19b through NdeI and Xho 1 restriction endonuclease sites. The resulting plasmid containing WSP was transformed into the bacterial strain BL21 (DE3).The transformed cells were utilized to grow and express the histidine-tagged and the protein was purified using Ni-NTA affinity chromatography.SDS/PAGE gel analysis has shown that the protein was 69% pure and has approximate subunit molecular weight of 25 kDa. The protein purification is completed in one day and 1 liters of culture produced approximately 18 mgs of protein, an improvement on the previous protein expression and purification.

Keywords: Wolbachia surface protein (WSP), Apoptosis, Protein expression and purification, E.coli strain BL21 (DE3), Ni-NTA affinity chromatography. 

Title: Cloning, Expression, and Purification of Histidine-Tagged Wolbachia surface protein

Author: Uday Jayaramaiah, Hariprasad T P N, Deekshith Kumar N, Deepak K J, Puttaraju H P

International Journal of Life Sciences Research

ISSN 2348-313X (Print), ISSN 2348-3148 (online)

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Abstract: The aim of the present study was to investigate the effect of culture filtrate of cyanobacterium (Lyngbya.sp) with coir pith on seed germination of Vigna radiata L. in ASN III  media. The impact of seed germination was  analysed at different concs.(6.25, 12.5, 25, 50, 75 & 100%) and at various time intervals (24, 36 & 48 hrs) on 0^th day and 30^th day. Similarly the physicochemical parameters (TDS), biochemical parameters (chlorophyll a, protein, amino acid) and morphometric parameter (radicle length) were also performed on 0^th and 30^th day. The 30^th day study revealed that the biochemical parameters (protein, amino acids and chlorophyll a) exhibited significant variations during germination in combined treatment when compared to control. The  chlorophyll a content was high at 25% conc. while the protein levels were reduced and amino acid levels increased in combined treatment when compared to control. The TDS level was decreased with cyanobacterial treatment at 6.25% conc. when compared to control. The germination percentage was maximum in combined treatment at 36 hrs. and 48 hrs. in lower conc. when compared to other treatments while the radicle length was highly enhanced in combined treatment with cyanobacterium and coir pith when compared to other treatments. Thus, the outcome of this study results showed that the combination of cyanobacterium and coir pith in culture filtrate reduced the TDS content by converting the inorganic ions present in the filtrate into nutrients for growth which in turn promoted  better seed germination. 

Keywords:  Lyngbya sp., Coir pith, ASN III media, seed germination, Vigna radiata L..

Title: The effect of culture filtrate of cyanobacterium (Lyngbya.sp) with coir pith on seed germination of Vigna radiata L.

Author: Tharani.G_, Sowmya.R, Malliga.P

International Journal of Life Sciences Research

ISSN 2348-313X (Print), ISSN 2348-3148 (online)

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Abstract: Siderophores are small protein molecules that can easily bind to ferric iron. As a chelating agent they transport iron molecules inside the bacterial cell for various biochemical reaction. At present studies characterization and Optimization of few siderophores producing bacteria from the soil samples collected from Salem district, Tamil Nadu. The siderophores production was assayed qualitatively and quantitatively through Chrome Azural S and the results showed positive for the strains PSF01 and PSA02 that grown in succinate medium. Further characterization and optimization results revealed that both the bacterium has the ability to yield siderophores in the optimum condition of P^H 6 at temperature 37º c with xylose as a carbon source and NH ₄SO₄ as a Nitrogen Source. Estimation of siderophore production in different media using maximum % siderophore unit (85%) King’s B Medium Fe content in different media using maximum % of siderophore unit (80%) in Nutrient broth medium.  The antagonist activity of the siderophore producing Pseudomonas spp against fungal pathogen Fusarium oxysporum. This result showed that Pseudomonas spp is a good producer of siderophore which can be usefully exploited as a biocontrol agent.

Keywords: Biocontrol, Pseudomonas spp, siderophore, Optimization, Fusarium oxysporum.

Title: Production and optimization of microbial iron chelators (Siderophore) byPseudomonas spp

Author: Nithyapriya. S, Lalitha. S

International Journal of Life Sciences Research

ISSN 2348-313X (Print), ISSN 2348-3148 (online)

Research Publish Journals

Abstract: The environment has been contaminated with organic and inorganic pollutants. Soil contamination was toxic metals, such as Cd, Pb, Cr, Ni, Cu, as result of worldwide industrialization has increased noticeably within past few years. Bioremediation is a biological process for cleanup of pollutants from the environment. In the present study, Bacillus cereus and Pseudomonas aeruginosa isolated from rhizosphere soil was used for bioremediation. These isolates were characterized for their ability of biochemical tests. Bacillus cereus and Pseudomonas aeruginosa were inoculated in Vignamungo.L plants by pots inoculation method and analyzed for the growth promotion efficacy in heavy metal polluted soil. The plant inoculated with isolates showed Plant height, root length and fresh weight, dry weight, as compared to non-inoculated control.Further analysis of leaves were carried out for the photosynthetic pigment analysis, in Vignamungo.L. Results of the study showed positive growth response of Vignmungo.L on heavy metal polluted soil in green house condition in plants. The study concluded that the heavy metal mobilizing PGPR could be used as effective inoculants for improving the phytoremediation in heavy metal contaminated soil as well as reclamation of heavy metal polluted soil. Phytoremediation is the process by which PGPR are stimulated to rapidly degrade the heavy metal contaminated soil to environmentally safe levels in soils. Such metal resistant PGPR, when used as bioinoculant or bio fertilizers, significantly improved the growth of plants in heavy metal contaminated soils. Application of PGPR processing metal detoxifying traits along with plant-beneficial properties is a cost effective and environmental friendly metal bioremediation approach.

Keywords: Bioremediation, Biofertilizer, Bioinoculants, Environment, Plant Growth Promoting Rhizobacteria.

Title: Effects of heavy metal contaminated soils using Plant Growth Promoting Rhizobacteria (PGPR)

Author: Santha kumari.R, Lalitha.S

International Journal of Life Sciences Research

ISSN 2348-313X (Print), ISSN 2348-3148 (online)

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Abstract: Michelia champaca L. belongs to family Magnoliaceae commonly known as Son Champa. It has various uses in traditional medicine, perfumery etc. The essential oil obtained from the flowers has useful application in perfumery and pharmaceutical industries. Traditionally it is being used in fever, colic, leprosy, after delivery protection and in eye disorders. There are several problems related to propagation of this plant such as low percentage of seed germination caused by alkaloids content in seeds that inhibit seed germination. The present study was conducted to develop a protocol for the shoot initiation of Michelia champaca L. The best result obtained from MS media supplemented with 2.5mg/l BAP, 1.0mg/l KN and 0.5mg/l IAA plus activated charcoal, which plays the significant role.

Keywords: Michelia champaca L.; Shoot initiation; Growth hormones; Phenolics; Activated charcoal.

Title: EFFECT OF GROWTH HORMONES ON SHOOT INITIATION OF MICHELIA CHAMPACA L.

Author: Rajshree Sinha, Ranjana Varma

International Journal of Life Sciences Research

ISSN 2348-313X (Print), ISSN 2348-3148 (online)

Research Publish Journals

Abstract: Exceedingly sensitive liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) with positive ion detection method was elaborated and validated to resolute prime compounds present in the active fraction of five various extracts of Benincasa hispida (Thunb.) Cogn. fresh fruit pulp. Five different polarity extracts of Benincasa hispida fresh fruit pulp crude were compared and screened for their identities and concentration of compounds present in the samples with authentic chemical β-sitosterol. Benincasa hispida a potential source for valuable nutrition and traditional medicines.

Keywords: LCMS Analysis, β-Sitosterol, Benincasa hispida, Bioactive compound.

Title: Liquid Chromatography Mass Spectrometry method in profiling of bioactive compounds in various extracts of Benincasa hispida (Thunb.) Cogn. fresh fruit pulp

Author: Vedha Balakumar, E. Rajesh, M. Gopalakrishnan, T. Sekar

International Journal of Life Sciences Research

ISSN 2348-313X (Print), ISSN 2348-3148 (online)

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Abstract: Trichoderma species are widely used as biocontrol agents for phytopathogenic fungi in agricultural fields. The occurrence of diseases such as damping off, Fusarium wilt and leaf spot, blights (Alternaria spp.) are important major diseases of chilli (Capsicum annum L.)  . Trichoderma  harzianum (MH333257) act as biocontrol agents against plant pathogenic fungi such as Fusarium oxysporum(RORK02),  and Alternaria spp. The microbial isolates were identified by microscopic analysis and confirmed through genetic DNA isolation and PCR analysis. Trichoderma harzianum were screened for antifungal activity against Fusarium oxysporum (RORK02), and Alternaria alternata and zone of inhibition was taken as an indicator by dual culture method. In this study chilli plants were harvested after 30 days of microbial inoculation and plant biomass, chlorophyll content and Enzyme activity were calculated. Trichoderma inoculated plants highly inhibited Fusarium.

Keywords: Alternaria alternata, Antagonistic activity, Biocontrol, Fusarium oxysporum, Trichoderma harzianum. 

Title: Biocontrol Potential of Trichoderma harzianum against Fusarium oxysporum and Alternaria alternata in Chilli plants

Author: R.PAVITHRA, S.LALITHA

International Journal of Life Sciences Research

ISSN 2348-313X (Print), ISSN 2348-3148 (online)

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Abstract: The role of the O-acetyl N-glycolyl neuraminic acid (O-acetyl NeuGc) specific lectin isolated and purified from the hemolymph of the freshwater crab Travancoriana charu in the innate immunity of the crab was documented using mammalian erythrocytes as antigenic stimulants. The erythrocytes when injected into the hemocoel regardless of their agglutinability and concentration, manifested increase in HA titer at varied time intervals.  Augmentation in hemagglutinating activity, post administration of erythrocytes indicates an increase in the circulating lectin.  Faster clearance of erythrocytes coated with lectin than the uncoated ones, indicates the role of lectin in the process of elimination of foreign pathogens.  Lectins played an eminent role in enhancing hemocyte mediated hemolysis, functioning as an opsonin. 

Keywords: Hemagglutinin, lectin, induction, clearance, Travancoriana charu.

Title: Biological role of the hemolymph lectin of the freshwater crab Travancoriana charu

Author: Sheeja V U, Basil Rose M R

International Journal of Life Sciences Research

ISSN 2348-313X (Print), ISSN 2348-3148 (online)

Research Publish Journals