A Comparative Study on Secondary Metabolite Producing Microbes Isolated From Rhizospheric and Non Rhizospheric Region of Alove barbadensis

Chhavi Singh, Pratibha Teotia

Abstract: The aim of the study was assess the antibacterial effect of plant extracts and their synergistic antibiotic and non antibiotic drugs against E.coli and S.aureus. The present study is carried out by the isolation and characterization of Rhizospheric and non-Rhizospheric region of Aloe Vera soil and comparative analysis of production of secondary metabolites against two bacterial pathogens (E. coli, S. aureus).The secondary metabolites will not involve in the growth of the cultures but they will be resultant of the primary metabolites. So, after isolation, the antibiogram analysis revealed the activity of isolates and further all were characterized through Bergey’ manual. The study was started from isolation, Primary Screening, culture characterization, pure culturing, antibiogram test, secondary screening, secondary metabolite extraction, and at last antibiogram test of secondary metabolites. Thereby, our results indicate the possibility of using these extracts in the treatment of bacterial infections, and the results of this study was encouraging, despite the need for clinical studies to determine of the real effectiveness and potential toxic effects in vivo These results were revealed the importance of plant extracts when associated with antibiotic and Non-antibiotic drugs in control of bacteria. Keywords: Rhizospheric, Non-Rhizospheric, Metabolites, Antibiogram. Title: A Comparative Study on Secondary Metabolite Producing Microbes Isolated From Rhizospheric and Non Rhizospheric Region of Alove barbadensis Author: Chhavi Singh, Pratibha Teotia International Journal of Healthcare Sciences ISSN 2348-5728 (Online) Research Publish Journals

Vol. 4, Issue 1, April 2016 – September 2016

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A Comparative Study on Secondary Metabolite Producing Microbes Isolated From Rhizospheric and Non Rhizospheric Region of Alove barbadensis by Chhavi Singh, Pratibha Teotia