Abstract: The effect of increasing saline stress on peroxidase enzyme was investigated in two varieties of rice (Pokkali), Oryza sativa var.V1 and Oryza sativa var.V2. The seeds were germinated in Hoagland’s solution containing 50mM, 100mM and 150mM NaCl. Samples were taken out at 24 hour intervals for 10 days and enzyme and protein assay was done. It was found that the peroxidase activity increased in both Oryza sativa var.V1 and Oryza sativa var.V2 with the increase in the duration and the concentration of NaCl. The percentage of enzyme activity over control was also found to be increasing in both varieties. The percentage of peroxidase activity over control was found to be more in Oryza sativa var.V1. The amount of protein was found to be more in 150mM NaCl treated seedlings and the amount decreases as the duration increases in both. Enzyme activity in units/mg protein was calculated. Among these two varieties, Oryza sativa var.V1 was found to be more tolerant towards salt stress. These results indicated that salt-tolerant varieties exhibit protection mechanism against increased radical production by maintaining the activity of antioxidant enzyme peroxidase.
Keywords: Oryza sativa, Pokkali, salinity, antioxidant enzymes, peroxidase.
Title: A COMPARATIVE STUDY ON THE EFFECT OF SALINE STRESS ON PEROXIDASE ACTIVITY IN TWO VARIETIES OF GERMINATING RICE (POKKALI) – Oryza sativa var.V1 and var.V2
Author: Nicy Joy, Anand P S, Dr. Suprabha G Nair
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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