Abstract: Umbilical cord blood transplantation has limitations due to lesser number of Hematopoietic Stem Cells (HSCs) in the graft, needed for efficient hematopoietic reconstitution. Besides that, problems in getting an appropriate HLA match and incidence of severe GvHD are inherent issues. Ex-vivo CD34+ HSC expansion is one of the key strategies to increase HSC numbers to meet the recommended dosage requirement. Recent reports have demonstrated the plasticity of CD34+ HSCs that helps differentiate them into various cell types including renal, cardiac, liver, neural, mesenchymal and muscle cells. This makes CD34+ cells yet another novel cell type for regular use in regenerative medicine applications.
Objective: The objective of this research was to establish a cost effective ex-vivo expansion of HSCs using perinatal MSCs as a feeder layer while maintaining stemness and functionality.
Method: We used a mixture of Mesenchymal Stem Cells derived from three perinatal sources (Umbilical cord tissue, placenta and amniotic sac) as a feeder layer for Ex-vivo expansion of CD34+ HSCs pooled from multiple cord blood units. The media used contained different concentrations of cytokines. A mix of different MSCs release abundant growth factors and cytokines much required for the maintenance of phenotype and functionality of CD34+ HSCs during expansion.
Results: We obtained average 30 fold expansion of CD34+ HSCs. It was a cost effective process. The functional characterization confirmed the stemness of the expanded cells.
Conclusion: MSCs from perinatal sources is a good option as a feeder layer for the Ex-vivo expansion of CD34+ HSCs. This source of MSC is ontogenetically naive and can produce more amounts of cytokines for sustainable HSC expansion.
Keywords: Ex-vivo expansion, CD34+ HSCs, Mesenchymal Stem Cells, Perinatal sources, Regenerative Medicine, Co-culture.
Title: A Mixture of Mesenchymal Stem Cells from Perinatal Sources Supports Consistent Ex-vivo CD34+ Hematopoietic Stem Cell Expansion
Author: Sushilkumar Ramdasi, Chandra Viswanathan, Rohit Kulkarni, Abhijit Bopardikar, Raghwendra Kumar, Pradeep Somalapur, Poonam Pandey
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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