Abstract: Routine proteolytic assays employ different developing reagents where the clarity of the hydrolyzed zone is not very sharp and distinctly visible. We here present an easy and improved assay to detect the presence of extracellular protease on milk agar plates. In the following method, 10% tannic acid was flooded post incubation on agar plates and observed for any zone of hydrolysis. The result of the present study clearly defines the method to be highly sensitive for detection of any extracellular protease produced by bacterial cells.
Keywords: Protease, Tannic acid, Zone of hydrolysis.
Title: A Rapid Method for Detection of Proteolytic Activity Using Tannic Acid
Author: Jyoti Sharma, Dr. Nupur Mathur, Dr. Anuradha Singh, DeepaYadav, Aditi Agarwal, Prof. Pradeep Bhatnagar
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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