Abstract: The aim of the present study was to explore the leaf extract of Asparagus aphyllus belonging to family Liliaceae for its antimicrobial activity. The in vitro antimicrobial activity of the leaf methanol extract of Asparagus aphyllus and others extarct (ethanol, aqueous, ethyl acetate and chloroform) were assayed using the agar plate diffusion. Test microorganisms studied were Escherichia coli, Kiebsiella pneumonia, Proteus vulgaris, Pseudomonas aeruginosa and Staphylococcus aureus. Methanol extract (100μg/ml) inhibited the growth of all the test organisms and the maximum zone of inhibition against gram positive organism is Kiebsiella pneumonia (2.27 cm); against Staphylococcus aureus is (2.24 cm) and Pseudomonas aeruginosa (2.14 cm). Methanol, ethanol and aqueous extract showed minimum inhibitory concentration (MIC) of less than 6.25μg/ml against all test microorganisms. The minimum inhibitory concentration (MIC), above 12.5μg/ml showed by ethyl acetate extract for Staphylococcus aureus and less than 12.5μg/ml for Klebsiella pneumonia and Pseudomonas aeruginosa. The methanol extract exhibited antimicrobial activities followed by ethanol and aqueous. Ethyl acetate exhibited least antimicrobial activity.
Keywords: Asparagus aphyllus, Antimicrobial, Minimum Inhibitory Concentration, Staphylococcus aureus.
Title: Antimicrobial and Minimum Inhibitory Concentration activity of leaf extract of Asparagus aphyllus
Author: Priyanka Sharma, Deepa Goel
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
Research Publish Journals
