Abstract: This study aimed to evaluate the anticancer activity of bioinspired silver nanoparticles (Ag-NPs) and gold nanoparticles (Au-NPs) againstMCF-7 cells. Both Ag-NPs and Au-NPs were biologically synthesised using pteridophytes. In this work, synthesis of nanoparticles, characterisation and potential anticancer activity has been reported. Marsilea belongs to pteridophyte division can reduce HAuCl4 solution to Au-NPs and AgNO3to Ag-NPs due to the presence of secondary biological metabolites. Phytochemical examination reveals the presence of tannins, phenols, coumarins, flavonoids, anthro-quinones, terpenoids, saponins. These phytochemicals are responsible for the transformation and act as capping, reducing and stabilising agents. Formation of-NPs and Au-NPs were ascertained from UV-vis spectrometry, FTIR and XRD. The UV-visible study revealed the surface plasmon resonance. FTIR spectra confirmed the participation of functional groups. XRD reveals the peak positions and broadening, which indicates the size of particles. In both case sharped sharp peak was observed at 38° among 2θ values, the size distributions of Ag-NPs and Au-NPs were calculated by using Scherrer’s formula. The cytotoxic potential of both SNPs was assayed against MCF-7 cells (breast cancer cell line) and HEK293 cell (human embryonic kidney cell line) by MTT assay. Results suggested that the NPs from Marsilea inhibits explicitly the viability of cancerous cell only and are non-cytotoxic to HEK293 cells in the tested concentration range. Based on these descriptions we can speculate that synthesised NPs may be used as promising anticancer agents, as the inhibits the viability of human breast cancer (MCF-7) cells and not detrimental to standard cell line.
Keywords: Silver Nanoparticles, Gold Nanoparticles, Aquatic pteridophytes, Marsilea sp., MCF-7 cells.
Title: Aquatic fern (Marsilea sp.) assisted synthesis of Silver and Gold nanoparticles and evaluation of their anticancer properties
Author: Sabiha Zamani, Danish Idrees, Samudrala Gourinath, Anal K. Jha
International Journal of Interdisciplinary Research and Innovations
ISSN 2348-1218 (print), ISSN 2348-1226 (online)
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