Abstract: Arsenic is the most noxious metalloid affecting the aquatic organism by inducing oxidative stress and histopathological abnormalities in vital organs. Bihar is emerging as the hot spot to the arsenic toxicity next to West Bengal in India. Most of the districts of Bihar have the prevalence of arsenic in the ground water. In the present study the freshwater air breathing fish Clarias batrachus (Linn.) has procured from Phulwarisharif Fish farm, Patna and acclimatized in the laboratory for 15 days. 96 hours and 48 hours LC50 of As2O3 for fish was calculated as per standard APHA method. The fishes were exposed to 2.5 ppm, 5.0 ppm and 7.5 ppm of As2O3 for one week. After the termination of exposure, the autopsy was done and the fish kidney were extracted and fixed in 10% NF and paraffin spread sections were prepared for light microscopy. The major histopathological anomalies incurred in kidney were – infiltration of neutrophils, fibrin and pus cells in the lumen of collecting tubules, oedematous, deposition in the lumen of PCT and DCT, infiltration of plasma cells in intertubular space, metastatic calcification of glomeruli etc. The findings of the present study shows that the nephrotoxicity generated in fish due to Arsenic is dose & duration dependent which ultimately lead to the renal failure of the fish. A close monitoring of the health of the fish is the need of hours for their conservation and enhancement of aquaculture sector of the State.
Keywords: Clarias batrachus, Nephrotoxicity, As2O3, LC50, renal tissues.
Title: Arsenic trioxide Induced Nephrotoxicity in Freshwater Walking Catfish Clarias batrachus (Linneaus, 1758): In vivo study
Author: Shrawan Kumar, Prakash Singh, G.B.Chand
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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