Abstract: The gram-positive and non-spore forming gut associated bacterium of Lactobacillus in freshwater cultivable fishes were investigated to assess the colonization pattern and characterization using standard microbiological procedures. We analyzed the bacterial diversity in the gastrointestinal tract of Catla catla, Labeo rohita, Cirrhinus mrigala, and Cyprinus carpio. The results show that the high diversity of bacteria were present and scarcely observed the bacterium Lactobacillus. The isolates LrMK2 and CmMK4 of Lactobacillus were selected and their morphological, biochemical and molecular characterization were identified. Further, it was selected for the evaluation of probiotic properties concludes that selective isolates LrMK2-Lactobacillus plantarum and CmMK4 – Lactobacillus equicursoris, which is potentially probiotics. The promising potential probiotic candidates were identified by 16S rRNA sequence and it was edited, aligned and submitted to NCBI
Keywords: Intestinal bacteria, Inland fishes, Lactobacillus sp., 16S rRNA profiling, Phylogenetic study.
Title: Assemblage and characterization of Lactobacillus associated with gut of freshwater cultivable fishes of South India
Author: Muthukumar.P, C.Kandeepan, R.V. Kalaimathi
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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