Abstract: In present investigation, the acute toxicity of Basic blue 3 dye for fresh water bivalve Lamellidens marginalis (7-8 cm) for 96 hours. The static bioassay was conducted at 40, 45, 50, 55, 60, 65, 70, 75, 80, 85 ppm on fresh water bivalve Lamellidens marginalis. The mortality rate was recorded and the LC0 and LC50 values for 96 hours were found at 40 ppm and 70 ppm respectively. The physico chemical parameters of test water like temperature, pH, total hardness, Dissolved O2, free CO2, inorganic phosphate and nitrate was measured. By examining the biochemical profile against control in different tissues like gill, mantle, hepatopancrease and gonad, the effects of LC0 and LC50 concentration were evaluated. There was highly significant depletion (p < 0.001) in glycogen content at both the concentration (LC0 and LC50) in all tissues. Maximum protein content depletion (p < 0.001) was in Gill at LC0 concentration compared to other tissues. While at LC50 concentration, decrease in protein content was significant in hepatopancrease, gill, mantle and gonad. The gill and hepatopancrease showed maximum decrease (p < 0.001) in lipid content at both the concentration (LC0 and LC50).
Keywords: Basic blue 3 dye, Biochemical content, Lamellidens marginalis, LC0, LC50.
Title: Assessment of biochemical effects of acute exposure of Basic blue 3 dye in fresh water bivalve Lamellidens marginalis
Author: Miss. Rupali Suresh Khandekar, Dr. Dipak Vishwanathrao Muley
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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