Abstract: The key to unravel most of the challenges associated with biodegradation and bioremediation of polluting substances may lie with autochthonous (indigenous) microorganisms present in polluted environments. Such bacteria can be isolated from stressed environments like sewage.They can also be found in soil habitat. Arthrobacter is one organism which is a common occurant in these habitats.These are pleomorphic organisms, exhibit typical rod to coccus morphogenesis and can degrade a wide range of organic & inorganic compounds, dyes and several pollutants. Forty five samples of sewage and soil were collected from Ulhas river and its basin .Organisms were isolated on Arthrobacter medium with pyridine. Preliminary identification of these isolates was done by Gram staining and biochemical characteristics. Further confirmation was done by phase contrast microscopy and their morphogenesis also was studied subsequently.Qualitative biodegradative screening of various organic as well as inorganic pollutants, pesticide, dyes and lignocellulosic materials, by these isolates, was checked. Twelve potent isolates were also studied for quantitative analysis of one dye (methyl orange), one inorganic pollutant (Chromium) and one organic pollutant(Acetonitrile. The activities of these isolates were constantly compared with those of Standard strains of Arthrobacter. Eighty five isolates were obtained from above mentioned samples. Morphology of thirty isolates matched with that of Arthrobacter. Sixteen isolates showed strong positive results of biochemical and morphogenetic characteristics .Identification was confirmed with 16s rRNA technique. All sixteen isolates could grow in presence of at least twelve out of nineteen contaminants.used for biodegradative screening. On quantitative analysis the percentage degradation was found to be upto 90% for dye, 76% for inorganic pollutant and 80% for organic pollutant. Thus Arthrobacter could go a long way in environmental cleanup of pollutants.
Keywords: Biodegradative, qualitative, morphogenesis, dyes, inorganic.
Title: Assessment of biodegrading potential of Arthrobacter isolated and identified from sewage and soil samples of Ulhas river basin
Author: Sandhya Mulchandani, Saraswati Patel
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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