Abstract: Drug resistant tuberculosis is becoming a major concern and has hindered the control of tuberculosis (TB) worldwide. What is the size of drug resistant-TB problem locally? Objective: To study mutation pattern of drug resistance tuberculosis isolates in Western Kenya. Methods: Sputum samples were obtained from TB patients and stained for acid fast bacilli and M. tuberculosis isolated from positive cases. Anti-tuberculous drugs resistance pattern and their genetic mutation patterns were screened by use of GenoType®MDRTBplus assay. Results: Among the 120 M. tuberculosis isolates from positive sputum smears, 102 (85%) isolates were sensitive to both rifampin (RIF) and isoniazid (INH) whereas 18 (15%) isolates were resistant to at least one of these drugs. Those that were resistance to RIF and INH were found respectively in 17/120 (14.2%) and 18/120 (15%) isolates whereas 17 (14.2%) were resistant to both RIF and INH. Genotypic multi-drug resistance was then confirmed in 14.2% (17/120) isolates. Genetic mutations in rpoβ 505-533, katG315 and inh-8 to -16 codons associated with resistance to RIF and INH in anti-tuberculous resistant isolates were found respectively in 17/18 (94.4%), 17/18 (94.4%), and 16 (88.9%). Conclusion: Findings from this study showed that the prevalence of anti-tuberculous drug gene mutation pattern among TB isolates was diverse with high prevalence in codons 505-533 and 315 of rpoβ and katG respectively.
Keywords: Anti-tuberculosis drugs, MTBDRplus, mutation patterns, inh gene, katG gene and rpoβ gene.
Title: Assessment of Genetic Mutation Patterns Associated With Anti-Tuberculosis Drug Resistance in Tuberculosis Isolates From Uasin Gishu County, Western Kenya
Author: Biegon RK, Nyamogoba HN, Ngeiywa M, Mining SK, Esamai F
International Journal of Healthcare Sciences
ISSN 2348-5728 (Online)
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