Abstract: Total fertilization failure (TFF) happens in 1–3% of total intracytoplasmic sperm injection (ICSI) cycles and may reoccur in sequent cycles. Regardless the high success rate with the applying of intracytoplasmic sperm injection (ICSI) , there's still a little number of couples who cannot get fertilized eggs after using ICSI . Six couples experiencing recurrent TFF or low fertilization rate (<10%) afterICSI were recorded in this study. Compared with the regularICSI group and the conventional ICSI-AOA group, the new AOA method using calcium ionophore, can seriously increase the fertilization rate from less than 10% up to approximately 50% in most cases. The normal distribution ofsperm-related oocyte activation factor phospholipase C zeta (PLCz1) in the sperms ofthe cases indicated the absence of an aberrant Ca signaling activation.
AOA method using calcium (Calcium ionophore A23187)was able to effectivelyimprove the fertilization rate in the majority of patients suffering from TFF. This AOAmethod had a potential therapeutic effect on those couples experiencing TFF, even after intracytoplasmic sperm injection(ICSI), which may overcome the severe fertilization deficiencies in patients with a repeated low fertilization or TFF.
Keywords: total fertilization failure, Calcium ionophore A23187, fertilization, assisted oocyte activation.
Title: Assisted Oocyte Activation Method (Ca ACTIVATION) Improves Fertilization in Patients with Recurrent Fertilization Failure
Author: Ayman S. El-Dorghamy, Bahgat A. El-Fiky, Mohammed H. Sayed Ahmad
International Journal of Healthcare Sciences
ISSN 2348-5728 (Online)
Vol. 10, Issue 1, April 2022 - September 2022
Page No: 251-257
Research Publish Journals
Website: www.researchpublish.com
Published Date: 30-August-2022
