Abstract: During the past five decades, dengue has evolved into one of the world’s most widespread infectious diseases and now constitutes a major global public health problem. Four closely related dengue virus serotypes (DENV-1, -2, -3, -4), are the causative agents of dengue disease. The spread of dengue virus (DENVs) has been attributed to massive unplanned urbanization, overpopulation, increasing global travel, and the inability to eradicate mosquito vectors. Dengue virus RNA genome contains 10,723 nucleotides translating a single ORF that encodes polyprotein precursor of 3391 amino acids residues , following which are series of post- translational proteolytic processing events generate the mature viral products. The viral poly protein has three structural and 7 non-structural (NS) proteins: C (core), prM (precursor to membrane), E (envelope), NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5. The multistep maturation process is helped by host proteases and dengue NS2B-NS3 protease by cleaving the viral polyprotein at certain sites into its constituent proteins. In this paper we report the self-cleavage activity of GST tagged NS2b-NS3 DENV type 2 protease.
Keywords: Auto-cleavage, dengue virus, protein purification, NS proteins.
Title: Auto-cleavage of GST Tagged Dengue Virus Type 2 NS2B-NS3 Protease
Author: Sushil Kumar, Seema Dwivedi
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
Research Publish Journals
