Abstract: Post operative wound infection is the commonest wound infection. It occurs if the integrity and protective function of the skin is breached. To identify the bacterial pathogens responsible for postoperative wound infections and determine their antibiotic sensitivity pattern. A total of 200 pus samples collected from surgical sites and immediately inoculated on blood agar and Mac conkey agar plate’s .Then the culture plates were inoculated at 37 0c for 24 hours. After incubation, isolates were identified by using gram staining and biochemical reactions. Sensitivity tests were performed on Muller-Hinton agar plate by Kirby-Bauer’s disc diffusion technique. This retrospective study was conducted over a period of six months from June 2014 to December 2014, at Narayana medical college and General hospital, Nellore. During this period, a total of 200 samples were collected, among these 136 (68%) showed positive growth. The most frequent isolate was staphylococcus aureus 38 (27.9%) followed by pseudomonas 28 (20.5%), E. coli 26 (19.1%). Antibiotic sensitivity test of the isolates showed that Ampicillin + sulbactam (100%) and vancomycin (95%) were the most effective antibiotics for gram positive bacteria and least effective for ofloxacin (25%). Gram negative isolates were E.coli (100%) , klebsiella (80%), citrobacter (60%), Acinetobacter(60%) were most sensitive to imipenum. Gram negative isolates were E.coli (60%) followed by klebsiella (80%), citrobacter (50%) and Acinetobacter (100%) were highly resistant to co-trimoxazole. Therefore antibiotic policy may help in the prevention and treatment of multidrug resistant pathogens in wound infection.
Keywords: Wound infections, staphylococcus aureus, pseudomonas, antibiotic sensitivity.
Title: Bacterial Profile of Post Operative Wound Infection and Antibiotic Sensitivity Pattern
Author: Meenakshi kante, N. Premanadham, P. Munilakshmi, P. Sreenivasulu reddy
International Journal of Healthcare Sciences
ISSN 2348-5728 (Online)
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