Abstract: Separation of any kind of protein from a complex mixture can be achieved by numerous methods. In this research experiment, SDS-PAGE technique was used. Samples of H2B and H3.3 protein were loaded in calculated manner and band images obtained after 1D electrophoresis were analyzed. Biomarker was taken as a reference to explore the migratory pathway followed by the sample protein. Basically, the impact of Bisphenol A (BPA) on protein in stimulated human cancer cells was governed. It was concluded that BPA induce mutation in histone protein by altering its molecular mass which depicts a correlation between BPA and cancerous cells.
Keywords: Biomarker, Bisphenol A, human cancer cells, gene profiling, breast tissue mutation.
Title: Biomarker Discovery in Bisphenol A Stimulated Human Breast Cancer Cells
Author: Richa Kaushal
International Journal of Healthcare Sciences
ISSN 2348-5728 (Online)
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