Abstract: Though post-mortem preserved sperms were shown to be useful in developing restoration procedures, there is a limitation in use due to their quality. Cryoprotectants currently under use are known to play a key role in retaining sperm quality but their impact on sperms under post-mortem is yet to be explored. Sperm motility is considered as the one of the most important quality to attain fertilization success. Chemical quality and microbial growth of stored semen decides the sperm quality. Hence the present study is carried out to study the changes in semen pH and microbial load in Cyprinus carpio post – mortem preserved directly or in suspension with Dimethylacetamide (DMA). This study showed that no significant change occurs in semen pH upon post – mortem preservation without / with DMA for upto 90 days compared to the control group maintained at room temperature. Further at PMP temperatures microbial growth was found to be kept significantly low indicating that preservation procedures including DMA utility did not induce any microbial contamination.
Keywords: C. carpio, Post-Mortem Preservation, Dimethylacetamide, Semen pH, Microbial growth.
Title: Changes in semen pH and microbial load of post-mortem preserved Cyprinus carpio suspended in Dimethylacetamide
Author: D. Sai Vinathi, V. Kalarani
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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