Abstract: The current study presents investigations on the characteristic of protein in different accessions of Urginea indica/ Drimia indica belonging to family Hyacinthaceae is a large polytypic genus of bulbous herbs. Different accessions of Urginea indica were collected from different regions of India like Ramanagar, Rangantitu, Gopalaswamibetta, sitampoondi, kerala and used for study. We have conducted our study using bulb part of the plant. Bradford method was used for estimation of protein. Highest protein was found in Rangantitu accession of Urginea indica around 0.88µg/ml. In Gopalaswamibetta accession the protein concentration was found to be 0.83µg/ml, Sitampoondi accession 0.75µg/ml, Ramanagar it was 0.71µg/ml and lowest protein concentration was seen in Kerala accession nearly 0.56 µg/ml. Native 12% poly acrylamide gel electrophoresis was carried out for characterization of protein in different accessions of Urginea indica. To see the diversification among species, three species of Urginea; Urginea indica, Urgnea wightii and Urginea polyphylla were taken into consideration for 12% native and SDS-PAGE.
Keywords: Protein, Native and SDS-PAGE, Urginea.
Title: CHARACTERIZATION OF PROTEIN IN DIFFERENT ACCESSIONS AND SPECIES OF URGINEA, HYACINTHACEAE
Author: Banani Misra, Hanumantha Rao, Shiva Kameshwari M.N.
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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