Abstract: All living organisms are inseparably interdependent with natural environment and interact with each other. UV-B radiation is a part of sun’s energy which due to depletion of ozone veil penetrates the earth’s surface in an elevated level and disrupts the vital metabolic processes of plants and induces genetic instability, which will ultimately be translocated to various trophic levels. Considering the above forementioned risk of UV-B on plants, the present experimental work has been designed to study the effect of UV-B exposure on root meristems of Lentil (Lens culinaris Medik). Experiment was carried out in four sets viz. A, B, C and D. A,B,C sets were treated with UV-B radiations for 20min, 40min,and 60min, successively for 1 day, 2day and 3day. Set D remain unradiated being kept as control. After each irradiation, roots were cut off from seeds and fixed in Carnoy’s fixative (GAA: AA, 1:3) for 24 hours then transferred in 90% alcohol. With the help of squash technique cytological slides were prepared. Various types of chromosomal anomalies were reported however the more prevalent were unorientation, precocious chromosomes, stickiness, scattering, c-mitosis, non-synchronisation, laggards, bridges etc. It was observed that the chromotoxic effect of UV-B was exaggerated as exposure duration elevated as evident in 60 minute treated sets. Hence, it was concluded that the higher intensity of UV-B exposure duration is fatal and genotoxic for plant's genetic machinery and induces genome instability.
Keywords: UV-B, irradiation, chromosomal anomalies, genotoxic, Lens culinaris Medik.
Title: Clastogenic and Aneugenic Responses of Supplemental UV-B Radiation on Root Meristems of Lentil
Author: Asha Pandey, G. Kumar
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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