Abstract: In the present study, leaves of Ocimumbasilicum were extracted using n-hexane and dichloromethane as solvents in the ratio 5:1.
These crude extracts were subjected to different chromatographic techniques for isolation of pure compounds. The infra red spectrum of the isolated compounds were obtained and interpreted to know the functional groups. GC-Ms was also carried out for the identification of constituents compounds.
The results observed are strong and broad band for phenol due to O-H hydrogen bonded alcohol (3423cm-1), C-H stretching vibration for aliphatic hydrocarbon (2956cm-1) and bending vibration for alkane at lower frequency (1462cm), C-N aromatic tertiary amine (1367), C-O tertiary and primary alcohol, C-H out- of-plane bending vibration of alkene with band of 896.
We have C-H out-of-plane bending vibration of disubstituted and phenyl substitution aromatic ring. The bending in this range gives strong bands in the 800-1000cm. The absorption bands of 737, 721, 669 are assigned to these phenyl substitution on aromatic ring respectively.
There is a unique chemical characteristic of sulphur.i.e S-S formation bond on extended chain called Catenation (polysulfide and arylsulfide with bands of 470 and 418).
Keywords: Ocimumbasilicum, aromatic tertiary amine, dichloromethane.
Title: Colour Reaction of the Chemical Components of Leaves of Ocimum basilicum on Thin Layer Chromatogram
Author: Ogunniran Blessing Ifeoluwa, Prof. G.A. Olatunji
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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