Abstract: DNA Isolation is a necessary protocol in Molecular Biology Research. Comparative Optimized DNA Isolation Protocol for six accessions of Sweet Potato (Ipomoea batatas [L] lam.) in Nigeria using FTA Plant saver card and DNA Zol was carried out with the aim of establishing a suitable and effective DNA extraction protocol from the leaves of sweet potato by comparing the bands formed after amplification using 5 Simple Sequence Repeats (SSR) primers. The accessions (Mothers Delight, King J, Butter Milk, DanZaria, NR 8164 and 87/TIS0087) collected from the National Root Crop Research Institute (NRCRI) Abuja, Nigeria were used as plant materials. Young leaves from the tip of the main vine of 20 days old plants were obtained and subjected to FTA Plant saver card and DNA Zol extraction protocols. Polymerase Chain Reaction in a touch down fashion and Agarose gel electrophoris were then performed on the extracted DNA and the bands formed were viewed under U-V light. The result showed that although both the FTA Plant Saver card and DNA Zol extraction method were able to extract a considerable amount of DNA, the bands formed from the DNA extracted using DNA Zol extraction protocol were more intense and clearer than those formed from the DNA extracted through FTA Plant Saver Card. This is due to the fact that DNA Zol utilised a higher amount of total genomic DNA than FTA plant Saver card for amplification. The DNA Zol extraction protocol is therefore recommended as an effective and efficient DNA extraction protocol for the potato plant.
Keywords: DNA Isolation, FTA plant saver card, DNA Zol, Sweet potato, Protocol, Accession, Amplification, Bands, Polymerase Chain Reaction and Molecular Biology.
Title: COMPARATIVE OPTIMIZED DNA ISOLATION PROTOCOLS FOR SIX ACCESSIONS OF SWEET POTATO (Ipomoea batatas [L] Lam.) IN NIGERIA USING FTA PLANT SAVER CARD AND DNA ZOL
Author: Ubelejit Alfred, Godspower Ekeruo, Sunday Ameh, Stephen Amos
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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