Abstract: The experiment compared two methods of DNA extraction in Livingstone and Hausa potato. DNA zol and plant saver card were optimized, simplified and presented. The aim of the experiment was to establish a suitable protocol for the extraction of these plants in Nigeria, in order to save time, cost and energy which may be spent during DNA extraction process. 20 day old fresh leaves of Livingstone and Hausa potato were extracted for the experiment. DNA extracted from four different accessions were amplified using 10 SSR primers. The results indicated that only the DNA zol technique was effective in extracting sufficient amount of DNA from the plants. Even though DNA zol requires more leaf sample, it proved to be the best technique for the extraction of DNA from the leaf samples. FTA plant saver card was however unable to trap enough amount of DNA from the leaves. Therefore, DNA zol technique is recommended for the timely and quality extractions and amplification PCR using the outlined protocols in this article.
Keywords: Opimization, DNA extraction, FTA plant saver card, DNA zol, Protocols, Livingstone potato, Hausa potato, Plectranthus esculenthus, Plectranthus roduntifolius, Plectranthus species.
Title: Comparative optimized protocols for DNA extraction and purification using FTA plant saver card and DNA zol methods for Plectranthus species (Plectranthus esculenthus and Plactranthus roduntifolius) in Nigeria (Livingstone and Hausa potato)
Author: Inalegwu Bawa, Danjuma Yakubu, Catherine Danmaigona, Amos Stephen
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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