Abstract: Anoectochilus roxburghii (Wall.) Lindl. is a herbaceous epiphytic orchid that is found in the following locations: Yunnan, China; Assam, India; Bangladesh; Eastern Himalayas; Nepal;Western Himalayas; Sri Lanka; Myanmar; Thailand; and Vietnam.A.roxburghii plant provides valuable medicinal material. A. formosanus is a terrestrial orchid that is widely distributed in Taiwan and Fujian Province of China and in Japan. A.roxburghii has been used as a Chinese folk medicine for many years. However, few reports on its in vitro regeneration are available. Thus, expanding A. roxburghii(Wall.) Lindl. sources is important. In this paper, a rapid micropropagation and cell culture system was established for wild A.roxburghii harvested from Yunnan. We designed an optimized induction medium for protocorm-like bodies, and the medium comprised the following: Murashige and Skoog(MS) medium +2,4-dichlorophenoxyacetic acidat 1 mg/L + kinetinat 0.5 mg/L+1-naphthalene acetic acidat 1 mg/L+6-benzyladenineat 0.5 mg/L+1% agar+3% sucrose. This medium was tested and was shown to be equally suitable for shoot culture after 8 weeks. The culture was maintained up to 12 weeks. Other cultures were initiated from shoots inoculated on MS medium supplemented individually with different concentrations of hormones. Medium supplemented with 3% (w/v)sucrose was the best among all treatments.The major bioactive compounds of A.roxburghii and A. formosanus were polysaccharides, amino acids,and alkaloids, and these compounds were obtained from whole plants of both species. The dried and powdered extracts fromwhole plantswere obtained by ethanol extraction using Soxhlet method. When whole plants were subjected to ethanol extraction,the polysaccharide, amino acid, and alkaloid levelsin A.roxburghii were 5.2%,8.2%, and 3.8% and those in A.formosanus were 6%–8%,4.7%, and 7.6%, respectively.
Keywords: In vitro culture, Orchids, Anoectochilus roxburghii, Orchidaceae, Natural products, Anoectochilus formosanus.
Title: Comparative Study of the Chemical Components of Anoectochilus Roxburghii and Anoectochilus Formosanus Tissue Culture
Author: N. M. Reda Refish, C.H. Fu, M.G. Wang
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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