Abstract: Amylase has been exploited in textile and detergent industries for desizing and stain removal due to having its hydrolytic features lead to breakdown of glycosidic linkages in starch into low molecular weight products such as glucose, maltose, maltotriose units, limit dextrins. Amylase immobilization is also lead to increase thermal and storage stability when it was bound onto bovine serum albumin (BSA) to prepare n enzyme bound nanoparticles. In present work, we were extracted amylase form Pearl millet seedlings and bound in BSA nanoparticles with four Almond Oil, Jasmine Oil, Mustard Oil, and Olive Oil as naturally occurring emulsifier. These fabricated nanoparticles were used as bio-active laundry additives with different detergents named, Ariel matic, Surf excel liquid, Active wheel and Tide plus to test their stain removal competency. Selected strains in this study were comprised of industrial/domestic routine strains, namely, Acryl paint, Oil paint, Grease, Ink, Rust strains. These industrial/common domestic stains are tough to vanish from clothes and often require lethargic pre-washing practices. Hence, from this study, fruitful comparative analysis was carried out from this study and all strains are very well wash off if when washed with chosen detergents with the prepared amylase bound nanoparticles solution as compared to when washing was done with chosen detergents only. Among them, Ariel matic detergent with enzyme loaded bovine serum albumin nanoparticles was found to be have best washing results as bio-active system compared to other chosen detergent samples, followed by Surf excel liquid, Tide plus and the least effective was found to be Active wheel. Among the prepared enzyme bound BSA nanoparticles, Almond and Olive oil driven nanoparticles was showed excellent washing competency when mixed with Ariel matic while mustard oil nanoparticles with Ariel matic also showed comparable good washing effects. Furthermore, very good results were also found to be observed when stained cloth was washed with Almond and Olive Oil driven nanoparticles with Surf Excel liquid. While, fairly good results were shown by Jasmine oil driven and Mustard Oil driven nanoparticles followed by Almond and Olive Mustard Oil driven nanoparticles with Active wheel and tide plus. As well as, least washing efficiency was found to be for Jasmine oil driven BSA nanoparticles.
Keywords: Acryl paint; Oil paint; Grease; Ink; Rust strains; Almond oil; Olive oil; Jasmine Oil; mustard oil; BSA nanoparticles; Detergents.
Title: Cost-Effective Washing Study of Acryl paint, Oil paint, Grease, Ink, Rust Stained fabric with Bio-active Emulsified Pearl millet Amylase loaded BSA nanoparticles as Laundry additives
Author: Kirti Rani, Sunidhi Kant, Sherya Goyal, Chandrika Gupta
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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