Abstract: The carbapenems are used as drugs of last resort for treatment of severe infections associated with the Extended spectrum beta lactamases (ESBL). The emergence of Metallo-beta lactamase (MBL) producing bacteria is a threat to the efficacy of these drugs due to the broad spectrum of activity of the MBLs on carbapenems and other beta lactams. This study was undertaken to detect MBL among imipenem resistant isolates from the Great Kwa River and the antibiogram. A total of 30 surface water samples were randomly and aseptically collected from three sites of Great Kwa River and transported to the laboratory within 18h for analysis. The surface plating technique was used to isolate bacteria from these samples after a 5-step ten fold serial dilution. Following standard guidelines for antibiotics susceptibility testing, the imipenem resistant isolates were subjected to double disk synergy test (DDST) and combined disk test (CDT) for detection of MBL. From a total of 42 imipenem resistant bacterial isolates, 18(42%) showed MBL production using the DDST and CDT phenotypic methods. With the DDST, 51% of the isolates were positive for MBL production while 71% showed MBL production with the CDT. There was no significant difference between these phenotypic tests (p>0.05). The species frequency of MBL production among the isolates were E.coli (5%), Citrobacter sp (5%), Aeromonas sp (11%), Salmonella sp (5%), Plesiomonas sp (16%) and Proteus sp (16%). All the MBL producing isolates were resistant to at least three antibiotics; the number of antibiotics all the isolates showed resistance include: Salmonella sp (7), Aeromonas sp (0), E.coli (8), Serratia sp (0), Plesiomonas sp (2), Proteus sp (0). The widespread emergence and spread of MBL producing bacteria is a threat to human health because of the multiple antibiotics resistance nature. Early detection of these isolates and regulation of carbapenem usage should be implemented.
Keywords: imipenem resistant isolates, Extended spectrum beta lactamases (ESBL), drugs, treatment.
Title: DETECTION OF METALLO-BETA-LACTAMASE AMONG IMIPENEM RESISTANT BACTERIAL ISOLATES FROM GREAT KWA RIVER, NIGERIA
Author: Andy, I. E, Archibong, C. P.
International Journal of Healthcare Sciences
ISSN 2348-5728 (Online)
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