DETECTION OF MYCOTOXIN PRODUCE BY ENDOPHYTIC FUNGI

Osman M. O. El – Maghraby, Yassmin M. Shebany

Abstract: The endophytic fungi can  be considered the symbiosis relationship between plants and fungi,  where the major features of mutualistic symbiosis include the lack of destruction of most cells or tissues, nutrient or chemical cycling between the fungus host, enhanced longevity and photosynthetic capacity of cells and tissues under the influence of infection,  enhanced survival of the fungus. But endophytes may produce mycotoxins, or otherwise alter host physiology and morphology of the plants. In this study, studied the ability of endophytic fungi to produce mycotoxin, and identified the mycotoxin. Can be isolated the endophytic fungi from three plants and chosen the dominant fungi and tested for producing mycotoxin and identified the mycotoxin. The chosen fungus is chaetomium sp. Firstly study The effect of Chaetomium species filtrate on Artemia salina to detect the ability of this genus to produce toxins and identify these toxins. Eight Chaetomium species out of 28 isolates tested had toxicity (high or moderate) to brine shrimp larvae, toxigenic Chaetomium species belonged to five species and these are Chaetomium atrobrunneum, C. causiiforme, C. gelasinosporum, C. globosum and C. hexagonosporum. Scondly identification of mycotoxin, sterigmatocystin was detected in different toxic extracts (8 extracts) of toxigenic Chaetomium species (5 species) in concentration varied between 12-13 µg / 50 ml medium. Four unknown compounds (yellow flouresent) at RF values 0.31, 0.4, 0.43 and 0.53 were detected in different toxic extracts.

Title: DETECTION OF MYCOTOXIN PRODUCE BY ENDOPHYTIC FUNGI

Author: Osman M. O. El – Maghraby, Yassmin M. Shebany

International Journal of Life Sciences Research

ISSN 2348-3148 (online), ISSN 2348-313X (Print)

Research Publish Journals

Vol. 2, Issue 4, October 2014 - December 2014

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DETECTION OF MYCOTOXIN PRODUCE BY ENDOPHYTIC FUNGI by Osman M. O. El – Maghraby, Yassmin M. Shebany