Abstract: Tuberculosis, one of the oldest diseases known to affect humans is caused by bacteria belonging to the Mycobacterium tuberculosis complex. Smear microscopy and culture forms the backbone of tuberculosis (TB) laboratory investigations in tertiary healthcare facilities which have a large number of cases and financial constraints. 1030 patients Sputum samples received in Microbiology laboratory in a tertiary care hospital during one year period was performed with Ziehl-Neelsen Acid Fast Staining, grading’s were as per RNTCP grading. Among 1030 patients, 130(12.62%) were AFB smear positive. All positive sputum specimens were concentrated by using Petroff’s method and inoculated in Lowenstein Jensen medium. The inoculated LJ medium was incubated at 37 C. The results were recorded. Mycobacterium tuberculosis and Non-Tuberculosis Mycobacteria were differentiated based on their colony morphology and other identification tests.
Keywords: Sputum smears microscopy, LJ medium, Conventional diagnostic methods, Mycobacterium tuberculosis, Non-Tuberculosis mycobacteria.
Title: Diagnosis of Pulmonary Tuberculosis Using Conventional Smear Microscopy and Culture Methods in a Tertiary Care Hospital
Author: SM.Nachammai, Dr.Mangayarkarasi.V MD.
International Journal of Life Sciences Research
ISSN 2348-3148 (online), ISSN2348-313X (Print)
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