Abstract: In vitro micropropagation of Oroxylum indicum (L) Vent was established from nodal explants cultured on MS medium supplemented with varied combinations of BA and TDZ. Nodal explants were inoculated on media supplemented with various cytokinins (BA/TDZ) in combination with or without NAA (0.5 mg/l) and AgNO3 (2 mg/l). Multiple shoot induction with maximum 5 shoots and shoot length of 5 cm was seen on MS medium containing only BA (1 mg/l) and AgNO3 (2 mg/l). For induction of roots, in vitro shoots were treated with IBA at various concentrations on half and full strength of MS basal medium. Maximum root induction exhibited on medium containing with IBA (2.5 mg/l) and AgNO3 (2 mg/l). The plantlets so generated were processed through hardening procedure for acclimatization and transfer to the soil.
Keywords: Micropropagation, Medicinal forest tree, Oroxylum indicum, TDZ, AgNO3
Title: Effect of Cytokinin and Auxin on Micropropagation of Oroxylum Indicum (L) Vent: A “Mountain Tree of India”
Author: Vilas R. Parmar, Yogesh T. Jasrai
International Journal of Life Sciences Research
ISSN 2348-3148 (online), ISSN2348-313X (Print)
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