Abstract: The purpose of this study was to examine the shoot regeneration and propagation ability of Ocimum basilicum L. through plant tissue culture technique. Callus formation was also observed in this study. Shoots, stems and roots of 8-week-old aseptic seedlings were used in this study. All cultures were incubated at a temperature of 25 ± 1ºC and illuminated with fluorescent light for 16 hours of light and 8 hours dark. Shoot and stem explants were found to be very responsive to produce maximum callus weight when cultured on Murashige and Skoog (MS) medium added with 2.0 mg/L Benzylaminopurine (BAP) and 1.0 mg/L Naphthalene Acetic Acid (NAA) with 2.20 ± 0.24 g and 2.01 ± 014 g respectively. Meanwhile, shoot regeneration was observed to be optimum when stem explant was cultured on MS medium supplemented with 2.0 mg/L and 0.5 mg/L. Tissue culture technique is an effective method in propagating Ocimum basilicum L. and its features are preserved. Due to the demand of this plant species by the consumers, this study suggests that in vitro propagation could be a suitable technique to promote mass supply of Ocimum basilicum L. in future.
Keywords: Ocimum basilicum L., tissue culture, explants, callus, regeneration, propagation.
Title: Effect of Explants and Growth Regulators on Callus Induction and Shoot Regeneration of Ocimum basilicum L. (Sweet Basil)
Author: Siti Zubaidah Lood, Nor Azlina Hasbullah, Muhammad Al-Amin Mazlan, Fadhlul Khaliq Ab Patah
International Journal of Interdisciplinary Research and Innovations
ISSN 2348-1218 (print), ISSN 2348-1226 (online)
Vol. 11, Issue 4, October 2023 - December 2023
Page No: 60-64
Research Publish Journals
Website: www.researchpublish.com
Published Date: 16-December-2023
