Abstract: Cold stress is known to disrupt mitochondrial metabolism of the right ventricle (RV) in rats. This study was designed to investigate the generation of reactive oxygen species (ROS) and antioxidant defense in cold stress in the presence of a natural polyphenol, grape seed proanthocyanidin extract (GSPE). Adult, late-adult and old male Wistar rats were categorized into six groups : 1) control (CON), 2) GSPE -treated (CON+PA), 3) Induced chronic cold stress (CCS), 4) GSPE-treated CCS (CCS+PA), 5) induced acute cold stress (ACS) and 6) GSPE-treated ACS (ACS+PA). Superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GR) activities along with glutathione (GSH) showed decreased activities in the RV of CCS group and extent was higher extent in the ACS group compared to their controls. These decreases were more effective in the old compared to adults and late-adults. On the contrary, thioredoxin reductase (TrxR) and catalase (CAT) activities were significantly increased under stress. The observed reduction in SOD, GPx and GR activities were concomitant with increased levels of hydrogen peroxide, superoxide, and nitric oxide levels in the RV of stressed rats. Total thiols (P-SH) significantly decreased as a function of age and chronic stress. The levels of HSP72 increased in the adult and old CCS group. Therefore, cold stress induces right ventricular oxidative stress as a function of age and notably with a greater magnitude during acute compared to chronic stress. However, these changes were alleviated in the GSPE-treated groups regardless of age.
Keywords: Ageing, Cold stress, Free Radicals, Grape seed proanthocyanidin, Right Ventricle.
Title: Effect of grape seed extract as a function of cold stress and ageing: A study on the redox state in the right ventricle of rat heart
Author: B. Jyothi, S. Asha Devi
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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