Abstract: All around the globe, studies are conducted to determine the effect of Wi-Fi on the quality of sperms both in vivo and in vitro. To determine the level of damage to the sperms in vitro, semen analysis, sperm chromatin dispersion test and ROS test was performed to map the motility, vitality, free radicals and also the fragmentation in the sperm samples which are exposed and which are not exposed to radiations emitted by Wi-Fi router. It was found after exposing sperm samples to Wi-Fi for long durations in a closed cabin placing sperm samples near Wi-Fi router can affect sperm quality to a great extent, reducing motility and vitality of sperms and causing high DNA fragmentations in sperms. On the other hand un-exposed samples kept far away from Wi-Fi source were in better condition both in terms of mobility, vitality and had fewer fragmentations. ROS test conducted on the core/raw semen samples revealed that the samples exposed for 180 minutes showed highest levels of oxidative stress displaying dark purple color in the ROS Agarose –N GEL tubes and samples subjected to Wi-Fi for 90 minutes showed high levels of oxidative stress displaying purple color and samples un-exposed to radiations showed low levels of oxidative stress and displayed light pink color. This study proved that sperm samples exposed to a Wi-Fi router connected to laptop or computer for long time causes devastating effects on sperms in vitro.
Keywords: Sperms, in vitro, DNA fragmentation, ROS, motility, vitality, Wi-Fi radiations, router, laptop, computer.
Title: Effect of Wi-Fi Radiations on Sperms in Vitro: Sperm DNA Fragmentation and ROS
Author: Omkar Pokharkar, Himanshu Patel, Vidisha Bhatt
International Journal of Healthcare Sciences
ISSN 2348-5728 (Online)
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