Abstract: This study was carried out to find the detrimental effect on human spermatozoa when exposed to alcohol/ethanol in vitro. For this study semen samples obtained from 12 healthy individuals were washed using the sperm preparation technique and each washed samples were divided into equal aliquots. Then ethanol at various concentrations of 60 mm and 120 mm were added to the aliquots labeled as test samples and samples without ethanol were considered as control samples. All samples with or without ethanol were incubated for 1 hour (60 minutes) at 370C. These samples when subjected to semen analysis after exposure to ethanol, it was found that samples added with 60 mm ethanol showed a decrease in motility and vitality, sperms in samples with 120 mm ethanol were almost static with minimum motility. Sperms in both test samples revealed degraded vitality. DNA fragmentation test or SCD when carried out it was revealed that ethanol caused DNA fragmentation in sperms and as the concentration of ethanol increased, DNA fragmentation also increased, motility of sperms decreased and morphology was affected to some extent such as the head and tail defects which was less in the control samples. The sample aliquots with 120 mm ethanol showed morphological abnormalities. The ROS test performed using Agarose-N-gel tubes on the unwashed/raw samples exposed to ethanol in different concentrations revealed the highest oxidative stress displaying dark purple color and unexposed samples showed light pink color indicating low oxidative stress. Reactive oxygen species can damage DNA bases and can cause lesions that block the progression of replication. This study proved that, alcohol when consumed can destroy sperm mobility, vitality and causes high fragmentation levels.
Keywords: Ethanol, sperms, DNA fragmentation, motility, vitality, ROS.
Title: EFFECTS OF ALCOHOL ON HUMAN SPERMATOZOA IN VITRO: SPERM CHROMATIN DISPERSION TEST AND ROS
Author: Omkar Pokharkar, Dr. Himanshu Patel, Madhuri Patel, Vidisha Bhatt
International Journal of Healthcare Sciences
ISSN 2348-5728 (Online)
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