Abstract: Several enzyme extracts, crude and purified, have been used in the decolourization and degradation of various dyes including crystal violet. But, not much research has been carried out to analyse the efficiency of synergistic effect of enzyme extracts from two different organisms with different enzymatic profile. This study attempted to evaluate the efficiency of Ganoderma lucidum and Pleurotus sp. MAK-II crude extracts, separately and together, on decolourization and degradation of crystal violet. The combined extracts of both fungi achieved better decolourization and degradation of CV dye (98%; 30°C; pH 4.5; 100 rpm) than the individual enzyme extracts. This was evident from the UV-visible spectroscopy analysis and FTIR analysis. The antimicrobial studies suggested that the dye degraded by combined extracts does not have significant antimicrobial activity against tested five soil bacterium and it does not pose any danger to soil microbe when released into environment. Further, the combined extracts yielded better sprouting and seedling growth on Phaseolus vulgaris L. allowing its usage in agricultural purposes.
Keywords: Crystal violet; Degradation and decolourization; Ganoderma lucidum; Pleurotus sp. MAK-II; Phytotoxicity.
Title: Enhanced decolourization and degradation of crystal violet by Ganoderma lucidum and Pleurotus sp. MAK-II crude extracts: antimicrobial and phytotoxicity analysis of product
Author: Raja Mohan Gopalakrishnan, Kalaichelvan Puthupalayam Thangavelu
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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