Enzymatic Detachment of Staphylococcus aureus Biofilm

Niraj A. Ghanwate, Yogesh B. Bele

Abstract: The power of Staphylococcus aureus to make biofilm is considered to be a serious virulence factor influencing its survival and persistence in both the environment and host. In Staphylococcus aureus biofilms, a number one explanation for persistence infections is because it is highly immune to immune defences and anti-microbial therapies. This study was aimed to gauge the antibiofilm effect of varied enzymes against biofilm formation by clinical isolates of Staphylococcus aureus. Within the present study total of 120 clinical specimens of pus samples were collected from hospitals and processed for isolation and identification of S. aureus. Growth was found in 96 specimens. Out of 96, 64 were found to be Gram-positive cocci and 32 Gram-negative bacilli. From 64 Gram-positive cocci 52 were Coagulase positive i.e Staphylococcus aureus and 12 Coagulase-negative. All Staphylococcus aureus isolates were further investigated for biofilm formation by the Tissue Culture Plate method. From 52 isolates 32 (62%) moderate biofilm forming, 16 (30%) weak biofilm forming and 04 (08%) strong biofilm forming. Biofilm formed by Staphylococcus aureus isolates was treated by 1% solution Amylase and Lysozyme enzymes to gauge the antibiofilm activity of enzymes for the detachment of biofilm. It was observed that Amylase and Lysozyme showed considerable antibiofilm activity against Staphylococcus aureus biofilm. All the strong and moderate biofilms produced by strains of Staphylococcus aureus were rendered non biofilm. Thus, these enzymes are useful as antibiofilm agents against biofilm formed by pathogenic Staphylococcus aureus.