Abstract: Objective: To assess the efficiency of combined different epidemiological marker systems for identifying and typing clinical multidrug-resistant Klebsiella spp. isolates according to biotyping, serotyping, its sensitivity & resistance to some antibiotics and bacteriocin typing as well as relation with spreading as nosocomial infection to improve the management of such outbreaks.
Methods: In this study one hundred-and four (104) Klebsiella strains were collected. They were selected from 1007 hospitalized patients at Sohag University Hospital. All Klebsiella isolates were subjected to susceptibility testing by disc diffusion method. Serotyping of Klebsiella spp. by slide agglutination test using the specific polyvalent antiserum (specific to 38 K-types) used. Monoclonal anti-Klebsiella. IgG2a was the immuonoglobulin type. Detection of bacteriocin sensitivity and bacteriocin typing was estimated by the inhibition pattern they form, against 8 reference bacteriocin producer strains using scrap and streak method.
Results: All isolates exhibited multiresistant patterns ranging from 4-12 antibiotics. 99 (95.1%) out of (104) tested strains of Klebsiella isolates shown positive agglutination reaction with polyvalent antibody resembling K. pneumoniae serotype. It was observed that most of the isolated strains of Klebsiella were sensitive to more than one of the bacteriocin producer strains 87 (83.6%). {P1}& {P8} bacteriocin producer strains have the broad spectrum of activity. Bacteriocin (Klebocin) typing of Klebsiella strains showed that 21 different combinations of bacteriocin sensitivity patterns were observed. Bacteriocin types {1}, {1,8},{1,3,8},and {1,6,8} were the most prevalent bacteriocin types.
Conclusion: Typing of multiresistant strains can possess a significant contribution in epidemiological studies. The combination of phenotypic markers such as biotyping and serotyping and bacteriocin typing was more discriminating than either method alone.
Keywords: bacteriocin, Klebsiella, Epidemiological studies.
Title: Epidemiological studies using biotyping, serotyping and bacteriocin typing as marker systems to identify clinical multidrug-resistant Klebsiella spp
Author: Mandour S. A.
International Journal of Healthcare Sciences
ISSN 2348-5728 (Online)
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