Abstract: The purpose of this study was to examine the ability of in vitro propagation of Punica granatum L. through tissue culture system. The experimental design that was used in this research is Completely Randomized Design (CRD). Cultures were stored at a temperature of 25 ± 1ºC and light 16 hours of light, 8 hours dark. All data was recorded and analyzed using ANNOVA. Leaf explants were found to be very responsive when cultured on MS medium supplemented with plant growth regulators at concentration of 2.0 mg/L Benzylaminopurine (BAP) + 1.5 mg/L Napthalene Acetic Acid (NAA) and has been identified as the optimum medium for shoot regeneration. Leaf explant produced 1.33±0.32 shoots per explant at week 8. Whereas from stem explant, Murashige and Skoog (MS) medium added with 0.5 mg/L BAP and 2.0 mg/L NAA gave the highest shoot development with 1.00 ± 0.28 shoots per explant Various combinations of auxin such as NAA and cytokinin such as BAP, at 1.5 mg/L was used for a complete regeneration of Punica granatum L. in vitro. In conclusion, in vitro micropropagation technique is a suitable alternative in producing new generation of Punica granatum L. and its features are preserved. It proves that plant tissue culture technology could be an alternative solution to achieve high quality of Punica granatum L., therefore could increase the crop production.
Keywords: Punica granatum L., tissue culture, micropropagation, explants, MS medium, growth regulators.
Title: Establishment of in vitro Micropropagation from Explants of Punica granatum L.
Author: Muhammad Al-Amin Mazlan, Nor Azlina Hasbullah, Siti Zubaidah Lood, Fadhlul Khaliq Ab Patah
International Journal of Interdisciplinary Research and Innovations
ISSN 2348-1218 (print), ISSN 2348-1226 (online)
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