Evaluation of antioxidant activity and GC-MS analysis of bioactive compounds present in leaf extract of Youngia japonica (L.)DC. from Chandigarh

Harsimran Kaur, Richa Puri, M.L. Sharma, Sana Khan, Rinku Jhamta

Abstract: The present study reports the phytochemical investigation as well as nutraceutical aspect of leaves of Youngia japonica from Chandigarh. Youngia japonica, commonly known as Oriental false hawksbeard, is a weed species of flowering plant belonging to family Asteraceae. In vitro antioxidant analysis of plant extract through three different methods viz. DPPH, H2O2 and Phosphomolybdenum assay shows significant results as compared to the ascorbic acid which is taken as positive control. Results indicate that plant has good potential of phenolic (82.60±0.0071 at 250µg/ml conc) and flavonoid (41.64±0.0612 at 250µg/ml conc) compounds as well it can be regarded as natural source of antioxidants. Gas chromatography–mass spectrometry (GC-MS) analysis of the methanol extract showed a total of 10 components out of which 9,12,15Octadecatrienoic acid,2,3bis[(trimethylsilyl) oxy]propyl ester, (Z,Z,Z) (RT=58.34%), psi.,.psi. Carotene, 3, 4 didehydro1,1', 2,2'tetrahydro1' hydroxyl1methoxy (RT=23.20%) and hexadecanoic acid, methyl ester (RT=3.64%) were the major constituents identified in the methanol extracts, respectively. Methanol extract showed the presence of several bioactive components and offers reference data for additional research of its active components.

Keywords: Phytochemical, antioxidant, bioactive, GC-MS, methanol.

Title: Evaluation of antioxidant activity and GC-MS analysis of bioactive compounds present in leaf extract of Youngia japonica (L.)DC. from Chandigarh

Author: Harsimran Kaur, Richa Puri, M.L. Sharma, Sana Khan, Rinku Jhamta

International Journal of Life Sciences Research

ISSN 2348-313X (Print), ISSN 2348-3148 (online)

Research Publish Journals

Vol. 7, Issue 1, January 2019 – March 2019

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Evaluation of antioxidant activity and GC-MS analysis of bioactive compounds present in leaf extract of Youngia japonica (L.)DC. from Chandigarh by Harsimran Kaur, Richa Puri, M.L. Sharma, Sana Khan, Rinku Jhamta