Abstract: Chromolaena Odorata and other associated species of Annona Squamosa have been reported be nematicidal. This research was intended at providing more information on the nematicidal activity of Chromolaena odorata and exposure the nematicidal action of Annona squamosa. Ten grams each of the powders were drenched in cold 100 ml of methanol for one week to produce 100,000 mg/kg stock solution, filtered and concentrated. One ml of nematode suspension that contained 60 eggs of M. incognita extracted with sodium hypochlorite, one ml of nematode suspension that contained 60 one week old second-stage juveniles dispensed into glass blocks that contained one ml of the extracts. Percentage inhibition and juvenile mortality were estimated. Dry powders were analyzed for Infrared and phytochemicals. Water extracts inhibited egg-hatch of Meloidogyne incognita (25-100%) and killed the juveniles (2.3-100%). Water extracts, 20,000 mg/kg, 30,000 mg/kg as from fourth day had 100% mortality of the juveniles. The IR revealed alcohols, amines, unsaturated/aromatic compounds and phenols. The phytochemicals identified were saponins, tannins, phenols, alkaloids, flavonoids, anthraquinones and cardenolides. Saponins were of the highest amounts followed by tannins and phenols. These botanicals were inhibitors of egg-hatch, lethal to juveniles of Meloidogyne incognita and might be attributed to the phytochemicals.
Keywords: Meloidogyne incognita, Egg hatchability, Larval mortality, Nematicidal activity.
Title: EVALUATION OF EGG HATCHABILITY AND LARVAL MORTALITY OF METHANOLIC EXTRACTS OF CHROMOLAENA ODORATA AND ANNONA SQUAMOSA ON MELOIDOGYNE INCOGNITA
Author: Kamatchi K., Nattuthurai N., Krishnamoorthy S.
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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