Abstract: For conservation of an endangered, horticultural and medicinally important orchid Coelogyne ovalis Lindl., an efficient protocol for its propagation was developed via asymbiotic seed germination. Eight months-old capsules of C. ovalis were inoculated aseptically on different nutrient media viz., Gamborg (B5), Knudson C (KC) and Mitra, with or without supplementation of plant growth regulators. Amongst the studied media, the best seed germination response (88.74%) was found on KC medium. The germination percentage was found to be enhanced in KC medium supplemented with 3 µM of BAP (6-Benzylaminopurine). For shoot induction, the seedlings were further cultured in medium supplemented with different concentration of BAP and α-Naphthaleneacetic acid (NAA). The best shooting was observed in medium augmented with 15 µM of BAP and 5 µM of NAA in combination. Optimum rooting frequency of regenerated shoots was achieved with 15 µM NAA and 30 µM phloroglucinol in the medium. The well-developed plantlets were hardened in a compost mixture. Genetic integrity of the micropropagated plantlets was ascertained using Start Codon Targeted polymorphism (SCoT) and Inter Simple Sequence Repeat (ISSR) markers. The present investigation on in vitro regeneration of C. ovalis ensures rapid propagation of plantlets for conservation of clonally stable plantlets.
Keywords: Coelogyne ovalis, Asymbiotic, Conservation, horticultural, Genetic integrity.
Title: Ex - situ multiplication of Coelogyne ovalis Lindl.: Nutrient optimization for asymbiotic seed germination and mass scale propagation of genetically stable plantlets
Author: Nutan Singh, Suman Kumaria
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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