Expression Profiling of Yield Related Genes in Banana

Shubhankar Tarafdar, D. Sailaja, Shubha Banerjee, Rakesh Meena

Abstract: Banana (Musa spp., family Musaceae) is one of the principle fruit crop of the world, which is cultivated and consumed in many countries. The gene expression profiling of the six subtractive suppression cDNA clones SSh1c03, SSh1f02, SSh1g06, SSh3e08, SSh2a02 and SSh2b11of the transcripts of zinc finger A20 and AN1 domain-containing stress-associated protein, putative nuclease HARBI1, E3 ubiquitin-protein ligase UPL1-like, thioredoxin H1- like, 9S-lipoxygenase 4 and nematode resistance protein-like HSPRO2 of banana (Musa acuminata subsp. malaccensis) respectively was studied in flower and leaf of Grand Naine (Control) and four local genotypes ( BAT, DRG, BHM and GA) of banana by semi- quantitative reverse transcriptase PCR approach. The genes belongs to four cDNA clones SSh1c03, SSh1f02, SSh1g06 and SSh3e08 among the selected transcripts were showed up- regulation in floral and leaf tissues of all the selected genotypes of banana may contribute in yield. The local genotypes of banana did not show any significant difference by comparing with the control in their expression.

Keywords: Banana, yield related genes, RT-PCR, expression profiling.

Title: Expression Profiling of Yield Related Genes in Banana

Author: Shubhankar Tarafdar, D. Sailaja, Shubha Banerjee, Rakesh Meena

International Journal of Life Sciences Research    

ISSN 2348-313X (Print), ISSN 2348-3148 (online)

Research Publish Journals

Vol. 6, Issue 3, July 2018 - September 2018

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Expression Profiling of Yield Related Genes in Banana by Shubhankar Tarafdar, D. Sailaja, Shubha Banerjee, Rakesh Meena