Abstract: The existence of genetic substructure in E. coli was revealed by Whittam and colleagues (1983) and was later confirmed by Chaudhuri and Henderson (2012). This research is the first to investigate the various phylo-groups that exist among amongst APEC strains from scavenging local chicken in Morogoro region of Tanzania based on the modern quadruplex method. All eight phylo-groups were detected among the APEC strains in varying percentages. Among the APEC isolates, 63.2%, 68.4%, 78.9%, and 73.7% were found to belong to phylogroups D and B2, C and F respectively, whiles isolates in groups A, B1 and E were 15.8%,10.5% and 5.3% respectively. There was a strong simultaneous occurrence of several iron acquisition genes with of structural gene for microcin iss thus (iss/iuCD), outer membrane protein(ompA) thus (ompA/sitEp). Iron acquisition genes and serum resistance genes recorded significant (P<0.05) differences between APEC and non-APEC strains and can thereby be used as markers for APEC isolates identification. The toxin and invasion genes, astA and ibeA which are normally recorded in minimal percentages were found to be significant determinants of APEC in the study. Funding: This work was supported by the intra ACP mobility scheme
Keywords: APEC, scavenging local chicken, revised phylo-grouping.
Title: EXTENDED PHYLOGENY AND PATHOGENECITY OF E. COLI IN SCAVENGING LOCAL CHICKEN IN MOROGORO MUNICIPALITY
Author: Emmanuel Odartei Armah, Huruma Nelweki Tuntufye
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
Vol. 10, Issue 2, April 2022 - June 2022
Page No: 44-54
Research Publish Journals
Website: www.researchpublish.com
Published Date: 13-June-2022
