Abstract: Beta-carotene is a terpenoid pigment that is highly valuable due to its nutritional benefit as a precursor of vitamin A and its antioxidant properties. Carotenoids forms one of the most important classes of plant pigments and play a crucial role in defining the quality parameters of fruit and vegetables. Fatty acids, the main components of edible oil, are usually converted to fatty acid methyl esters. β-carotene and fatty acids were extracted and identified from Sesamum indicum. UV-visible spectra analysis by observing the Plasmon peak. Paper chromatography and thin layer chromatography was carried out to confirm the presence of bioactive compounds. HPLC, Saponification test and iodine test was performed to identify the presence of fatty acid and the degree of unsaturation respectively. The present study concludes that the presence of beta carotene and fatty acids in the Sesamum indicum.
Keywords: β-carotene, fatty acid, Sesamum indicum, Saponification, Iodine test, HPLC.
Title: Extraction and Identification of β-carotene and Fatty Acid from Sesamum indicum
Author: S.Seethalakshmi, V.Sivapriya
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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