Abstract: Tyrosine ammonia lyase (TAL) is an enzyme that deaminates the amino acid L- Tyrosine to p-coumaric acid. This enzyme was extracted from the plant Santalum album Linn. This study is aimed to determine the role of TAL enzyme for the treatment of hyperpigmentation in man by using Zebra fish embryo as test animal. Extraction of TAL was based on the principle of separation of proteins on treatment with an organic solvent acetone followed by the standardization of enzyme assay for the detection of the enzyme TAL. The presence of the enzyme was confirmed by the formation of p- coumaric acid which was detected using UV- Visible spectrophotometer at 333 nm. The crude extract was partially purified using ammonium sulphate. The activity of TAL was found to be hindered after partial purification. Hence zebra fish embryos were treated with crude enzyme extract of TAL. There was considerable reduction in the pattern of pigment formation in Zebra fish embryos. Hence Santalum album was found to be a good source of TAL and thereby can be used to treat Hyperpigmentation in man as tyrosinase enzyme in man is responsible for the conversion of tyrosine to melanin pigment. The enzyme Tyrosine ammonia lyase converts tyrosine to p-coumaric acid and thereby reduces the formation of melanin pigment resulting in hypopigmentation. Recent report suggest that there is syntenic relationship between zebra fish and human genomes.
Keywords: Tyrosine ammonia lyase, Santalum album, Hyperpigmentation, Zebra fish, UV- Visible spectrophotometer.
Title: Extraction and partial purification of Tyrosine ammonia lyase from Santalum album Linn. A possible solution for Hyperpigmentation
Author: Sarina. P. Khabade, Radhakrishna. N, Triveni. B
International Journal of Life Sciences Research
ISSN 2348-3148 (online), ISSN 2348-313X (Print)
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