Abstract: Plant Protease Inhibitors (PPIs) are widely distributed in plants and plays a role in protecting plants from pest attack. In this study we screened plants to identify protease inhibitors against trypsin. Seeds/leaves were homogenized in bicarbonate buffer pH 9.0 (1ml/g tissue). The homogenate was centrifuged at 9,500 x g for 10 minutes at 40 C. The supernatant containing the soluble proteins was used for protease inhibition assay using Nα-Benzoyl-DL-Arginine-P-Nitro Anilide (BAPNA) as substrate. Out of the 20 plants screened 11 plants showed greater than 50% inhibition towards trypsin and out of these for six plants, this is the first report of presence of trypsin inhibitor. The extract from the leaves of Dalbergia latifolia showed the highest inhibition of 85.09±0.4%. The other plant extract showed greater than 50% inhibitory activity towards trypsin are Nephelium lappaceum (83.45±0.05%), Cochlospermum religiosum (79.23±0.04%), Anacardium occidentae (79.23±0.04%), Samanea saman (78.27±0.08%), Mucuna pruriens (71.34±0.06 %), Psidium gujava (69.20±0.09%), Alpinia calcarata (67.25±0.12%), Syzigium cumini (61.10±0.26%), Gliricidia sepium (56.90±0.09%) and Pterocarpus santalinus (52.49±0.01%). To our knowledge no trypsin inhibitor was reported from Dalbergia latifolia, Cochlospermum religiosumi, Psidium gujava, Alpinia calcarata, Gliricidia sepium and Pterocarpus santalinus. As lepidopteran larval gut is rich in trypsin-like serine proteases, identification of PPIs against trypsin will be helpful in utilizing them for the controlling the larvae of lepidopteran pests, in addition to other uses of trypsin inhibitors.
Keywords: Plant protease inhibitors, Trypsin inhibitors, Dalbergia latifolia, Cochlospermum religiosum.
Title: IDENTIFICATION OF PLANT EXTRACTS CONTAINING TRYPSIN INHIBITOR
Author: P.P. Remya, Kannan Vadakkadath Meethal
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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