Abstract: The present study was conducted to evaluate the possible protective effect of flaxseed oil, curcumin and their combination against GCs-induced osteoporosis in female rats. Healthy female albino rats (n=70) weighting (160-200 g) were divided into six groups; group I: (n=10) negative control, Groups (second-sixth) (n=60) rats were injected with glucocorticoids (dexamethsone sodium phosphate) (2 mg/kg.b.w/day) for 3 weeks daily. Second group: (n=15) untreated rats (positive control).Third group: (n=10) rats were fed diet containing flaxseed oil (15g/ kg diet). Fourth group: (n=10) rats were orally administered with curcumin (5mg/kg.b.w/day). Five group: (n=10) rats fed on diet containing flaxseed oil (15g/kg diet) and orally administered with curcumin (5mg/kg.b.w/day).Sixth group :(n=15) rats were treated with estrogen (25mg /kg.b.w/day). Data revealed that GCs exhibited significant decrease in body weight, femoral weight, calcium (Ca++) and phosphorus (P) concentration in serum and femur bone, vitamin D, serum osteocalcin (OC), activity of alkaline phosphatase (ALP) (p<0.001) for each, elevated in parathyroid hormone (PTH) (p<0.001). Treatment with flaxseed oil, curcumin and mixture showed a significant elevation in Ca++, P+++, Mg++ in serum and femur, vitamin D, OC, ALP and decrease in PTH (p<0.01) compared with untreated group. Histopathological examination showed features of osteoporosis (cracks - wide Haversian canals - thin trabeculae).Treatment with the present substances preserves normal structure of both types of bone, which was more evident in combined treatment. It was concluded that, regular utilization of flaxseed oil and curcumin as an adjuvant supplement during prolonged Gcs therapy to protect against osteoporosis.
Keywords: Curcumin, flaxseed oil, osteoporosis, rats, glucocorticoids.
Title: Impact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Rats
Author: Turkyah J. Al-Bogami, Maryam A. Al-Ghamdi , Etimad A. Huwait, , Jalaluddin A. Jalal, Reem AL-Wafi, Bushra S. ALSahafi, Moselhy SS
International Journal of Life Sciences Research
ISSN 2348-3148 (online)
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