Abstract: Breast cancer is the second leading cause of cancer deaths in women worldwide. A number of strategies for the cure of breast cancer have been described such as surgery, chemotherapy and radiation; still, therapeutic effectiveness is characteristically temporary and commonly lacking with progressive period. As a result, the requisite for rational methods to breast cancer therapy is required. Palmitic acid (PA) is the most common saturated free fatty acid and plays an important role in cancer. However, the mechanism underlying the effect of PA on breast cancer is still to be elucidated.
Experimental: In our study we have hypothesized that PA would have anticancer activity on MCF-7 Breast cancer cell line.
Results: Pamitic acid reduced cell viability in MCF-7 Breast cancer cells, apoptotic nuclear morphology of cells was observed by DAPI (4',6-diamidino-2-phenylindole) staining after 48 and 72 hrs treatment, comet assay showed the increase tail length and DNA fragmentation. Treatment with PA enhanced the expression of apoptosis-related proteins including caspase-3, 9, Bax and p53 whereas the expression of anti-apoptotic protein Bcl-2 was decreased.
Conclusion: Pamitic acid sensitizes breast cancer cells, induced apoptosis and may be considered for upcoming studies as a promising therapeutic compound for human breast cancer.
Keywords: Breast cancer, Palmitic acid, apoptosis, inflammation, therapeutic action.
Title: In vitro Assessment of cytotoxic and apoptotic potential of Palmitic acid for Breast cancer Treatment
Author: Md. Zafaryab, Khalid Umar Fakhri, Md. Asad Khan, Krishnan Hajela, M. Moshahid A. Rizvi
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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