Abstract: Nyctanthes arbor-tristis L. is commonly known as Coral Jasmine and Parijat, belonging to the family Oleaceae (Nyctaginaceae). It is one of the most versatile medicinal plants having a wide spectrum of biological activities. The present study aimed to develop an improved protocol for in vitro propagation through the culture of axillary bud and shoot tip explants from in vitro germinated seedlings. Murashige and Skoog (MS) medium as basal medium and supplemented with different concentrations and combinations of 6-benzyl amino purine (BAP), α- naphalene acetic acid (NAA), Kinetin (Kn) and indole butyric acid (IBA) were used in order to shoot induction, multiplication and rooting. The medium without any plant growth regulators (PGRs) were employed for in vitro seed germination. The axillary bud and shoot tip were excised from in vitro germinated seedlings and inoculated on initiation medium. The maximum percentage of shoot induction with 6.10 ± 1.28 of shoots was produced on the medium fortified with 4.44 µM/L of BAP and 2.68 µM/L of NAA. The well initiated shoots were sub cultured on fresh medium composed of different concentration and combinations of PGRs for further multiplication of shoots. The medium enriched with 8.07 µM/L of BAP and 3.76 µM/L of NAA was found to be efficient for maximum percentage of shoot multiplication with the highest number of shoots (21.10 ± 1.85) and mean shoot length of 10.30 ± 1.41cm. The effective root induction with maximum number of roots was obtained on the half strength MS medium supplemented with 4.92 µM/L of IBA. The well rooted healthy plantlets were hardened and establish successfully with 95% of survival. This protocol may be useful for the large scale production of this plant and other medicinal plants.
Keywords: Nyctanthes orbor-tirstis, in vitro seed germination, axillary buds, shoot tip, in vitro propagation.
Title: In vitro Propagation of Nyctanthes arbor-tristis L. through Culture of Axillary bud and Shoot tip Explants from In vitro Germinated Seedlings
Author: Gandhi, K, Vijayakumar, C, Saravanan, S
International Journal of Interdisciplinary Research and Innovations
ISSN 2348-1218 (print), ISSN 2348-1226 (online)
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