Abstract: The objective of the current study was to comprehend the immune response in freshwater crabs, Paratelphusa jacquemontii. The purpose of this study was to demonstrate serum phenoloxidase (PO) activity. Different phenolic substrates were used to test the PO enzymes substrate affinity, and DL-3,4-dihydroxyphenylalanine (DL-DOPA) demonstrated the enzyme's highest substrate affinity. When the enzyme activity of serum with various DL-DOPA concentrations was evaluated, it showed that the enzyme activity was highest at 5 mM of DL-DOPA concentration. When the DL-DOPA enzyme activity was examined at various time intervals, it demonstrated stable activity at 10 min incubation duration. When the impact of various buffer ionic strengths on the oxidation of DL-DOPA by serum was evaluated, greater enzyme activity was found at a concentration of 50 mM Tris-HCl. Studying the impact of pH and temperature on the oxidation of DL-DOPA by serum, pH 7.5 and 25 °C were shown to have steady action. 10 mM phenylthiourea (PTU) inhibited the serum PO activity while trypsin, sodium dodecyl sulphate (SDS), and non-self molecule laminarin activated it. We also discovered the substances that in serum with strong PO activity inhibited or activated the enzyme in vitro, indicating for the first time an immunological function.
Keywords: Paratelphusa jacquemontii, innate immunity, phenoloxidase, inhibition and activation, enzyme activity.
Title: IN VITRO SERUM PHENOLOXIDASE ACTIVITY IN THE HEMOLYMPH OF FRESHWATER CRAB PARATELPHUSA JACQUEMONTII (RATHBUN, 1905)
Author: Rangasamy Shanthi, Mullaivanam Ramasamy Sivakumar, Somu Chitra
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
Vol. 12, Issue 2, April 2024 - June 2024
Page No: 12-24
Research Publish Journals
Website: www.researchpublish.com
Published Date: 07-May-2024
