Abstract: Orthodontic fixed appliances increase the risk of dental caries, often caused by Streptococcus mutans. Graphene Oxide (GO) exhibits antibacterial properties, and the thickness of nanomaterials may enhance their antimicrobial effectiveness. This study aimed to determine the bacteriostatic effect of a single and multiple layers of GO coating on stainless steel orthodontic bands on the inhibition of S. mutans. Sixty (60) Orthodontic stainless steel bands were divided into four groups - control group (uncoated brackets); experimental group 1 (treated with one (1) layer of GO coating); experimental groups 2 and 3 (treated with two (2) layers and three (3) layers of GO coatings respectively). Each experimental group was dipped in a 10mg/ml GO solution for two minutes per layer. The inactivation of S. mutans when exposed to the different layers of GO coating was assessed through modified agar well diffusion method. The results were analyzed using Guevara et al.,2005 index. Experimental groups 2 and 3 have shown a very active antibacterial effect against S.mutans with mean values of 38.00 and 21.5 mm respectively (very active > 19 mm). The least antibacterial activity against S. mutans was recorded in group 1 with a mean value of 9.43 mm (may be expressed as inactive < 10 mm). No zone of inhibition was observed with the control group of uncoated molar bands. The zone of inhibition diameter was compared and analyzed using Kruskal-Wallis test. Statistically, there was a significant difference among the groups with P value 0.000 <0.05.
Keywords: Graphene Oxide, Streptococcus mutans, Bacteriostatic effect, stainless steel orthodontic bands.
Title: Increasing Bacteriostatic Effect of Coating Stainless Steel Orthodontic Bands with Single and Multiple Layers of 10 Mg/Ml of Graphene Oxide against Streptococcus Mutans
Author: Yazan, Homsi, Phides Alcorta, Marie Gertrude Tuscano, Joseph Neil Acero, Michelle Karen Bunuan
International Journal of Thesis Projects and Dissertations (IJTPD)
Vol. 13, Issue 1, January 2025 - March 2025
Page No: 55-62
Research Publish Journals
Website: www.researchpublish.com
Published date: 08-February-2025
