Abstract: Jasmine (Jasminum sambac Ait.) is one of the plants which widely cultivated in Indonesia and used as a traditional medicine to cure diarrhea, fever, influenza, shortness of breath, and also act as an antibacteria. The objective of this study is to determine the antibacterial activity of ethyl acetate extract of jasmine flower against the growth of Streptococcus pyogenes and Escherichia coli. This study is a laboratory experimental study with a Randomized Post-Test Only Control Group design. Ethyl acetate extract of jasmine flower is diluted into four different concentration, such as 25%, 50%, 75% and 100%. The inhibitory potency test against the colony of S. pyogenes ATCC 19615 and E. coli ATCC 25922 was conducted in Laboratorium Kesehatan Provinsi Bali. The inhibition zone is measured by calipers. Data result were analysed by descriptive statistic. The ethyl acetate extract of jasmine flower inhibit the growth of S. pyogenes with the smallest inhibition zone at the 25% concentration (12,75 ± 0,500 mm) and the largest inhibition zone at 100% concentration (15,75 ± 0,500 mm). Ethyl acetate extract of jasmine flower inhibit the growth of E. coli with the smallest inhibition zone at 75% concentration (12,25 ± 0,500 mm) and the largest inhibition zone at the 100% concentration (19,00 ± 1,154 mm). The increased inhibition zone of the bacteria is directly proportional to the increase in extract concentration.
Keywords: ethyl acetate extract, jasmine flower, antibacterial activity, Streptococcus pyogenes, Escherichia coli.
Title: Inhibitory Potency of Ethyl Acetate Extract of Jasmine Flower (Jasminum sambac Ait.) Against the Growth of Streptococcus pyogenes and Escherichia coli
Author: Putu Ruzhira Ayu Risma Puspa, Ni Nengah Dwi Fatmawati, Ni Nyoman Sri Budayanti
International Journal of Healthcare Sciences
ISSN 2348-5728 (Online)
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